thermocellum. An ORF recently suggested as a putative pyruvate

kinase homologue, Cthe1955 [24], had relatively minimal MEK162 price expression during cellulose fermentation (Figure 4). C. thermocellum however has two copies of the gene encoding pyruvate phosphate dikinase (Cthe1253 and Cthe1308), which catalyzes the reverse reaction for conversion of pyruvate to PEP. This enzyme is suggested to play an anabolic role in gluconeogenesis and consistent with this, Cthe1253 is upregulated in stationary phase during growth on cellulose. Sparling and colleagues have proposed an alternate route for conversion of PEP to pyruvate via oxaloacetate and malate (Figure 4; Sparling, personal communication). Genes encoding all three enzymes in this alternative route, the gluconeogenic PEP carboxykinase (Cthe2874), malate dehydrogenase (Cthe0345), and malic enzyme (Cthe0344), were

expressed find more at high levels, suggesting that this putative pathway is active in C. thermocellum during growth on Selleck CP673451 cellulose. C. thermocellum contains two clusters of genes (Cthe2390-2393 and Cthe2794-2797) encoding the gamma, delta, alpha and beta subunits, respectively, of the thiamine-pyrophosphate (TPP) dependent pyruvate ferredoxin oxidoreductase (POR) which catalyzes the oxidation of pyruvate to acetyl-CoA. While all the genes in the Cthe2390-2393 cluster displayed maximal expression during cellulose fermentation, only a single gene in the Cthe2794-2797 cluster, encoding

Loperamide the TPP-binding beta subunit of the POR protein complex (Cthe2797), had high transcript levels which decreased in stationary phase (Figure 4). This is in contrast to studies by Sparling and colleagues who reported expression of Cthe2794-97 transcripts by RT-PCR during log phase of growth on alpha-cellulose with only weak expression of the Cthe2390-93 cluster [13]. However, the observed trends in gene expression may be due to differences in culture conditions between the two studies. While qPCR analysis was done with batch cultures in Balch tubes with no pH control [13], microarray analysis in this study was conducted with samples from controlled fermentations in bioreactors with pH control. Mixed-acid fermentation C. thermocellum encodes several genes involved in the mixed-acid fermentation pathways for conversion of pyruvate to organic acids (lactate, formate, acetate) and ethanol (Additional file 5, Expression of genes downstream of PEP). These include two putative lactate dehydrogenase genes (ldh; Cthe0345, Cthe1053) involved in conversion of pyruvate to lactate. Previous studies have reported detecting LDH activity in cell extracts of C. thermocellum [14, 25, 26] and RT-PCR analysis has shown expression of both ldh genes during cellulose batch and continuous fermentations [11, 13]. LDH, Cthe1053, cloned and expressed in E.