The peptide was slowly eluted with buffer B (3 mL, once), collected into a polystyrene tube and evaporated to dryness. The levels of β-endorphin were measured using a direct
β-endorphin EIA kit from Phoenix Pharmaceuticals (CA, USA). Statistical analysis The data were presented as means ± SD or SE. Student’s t test was used for von Frey hair test and a one-way analysis of variance (ANOVA) test was also conducted for immunohistochemistry and β-endorphin assay. Results Morphological changes of S-180 tumor mass around sciatic nerve and induction of neuropathic cancer pain As shown in Fig. 2A, S-180 cells grow https://www.selleckchem.com/products/bmn-673.html rapidly and embedded around the sciatic nerve in a time-dependent manner, which was confirmed by MRI scanning. On day 9 after inoculation, the sciatic nerve was {Selleck Anti-infection Compound Library|Selleck Antiinfection Compound Library|Selleck Anti-infection Compound Library|Selleck Antiinfection Compound Library|Selleckchem Anti-infection Compound Library|Selleckchem Antiinfection Compound Library|Selleckchem Anti-infection Compound Library|Selleckchem Antiinfection Compound Library|Anti-infection Compound Library|Antiinfection Compound Library|Anti-infection Compound Library|Antiinfection Compound Library|Anti-infection Compound Library|Antiinfection Compound Library|Anti-infection Compound Library|Antiinfection Compound Library|Anti-infection Compound Library|Antiinfection Compound Library|Anti-infection Compound Library|Antiinfection Compound Library|Anti-infection Compound Library|Antiinfection Compound Library|Anti-infection Compound Library|Antiinfection Compound Library|Anti-infection Compound Library|Antiinfection Compound Library|buy Anti-infection Compound Library|Anti-infection Compound Library ic50|Anti-infection Compound Library price|Anti-infection Compound Library cost|Anti-infection Compound Library solubility dmso|Anti-infection Compound Library purchase|Anti-infection Compound Library manufacturer|Anti-infection Compound Library research buy|Anti-infection Compound Library order|Anti-infection Compound Library mouse|Anti-infection Compound Library chemical structure|Anti-infection Compound Library mw|Anti-infection Compound Library molecular weight|Anti-infection Compound Library datasheet|Anti-infection Compound Library supplier|Anti-infection Compound Library in vitro|Anti-infection Compound Library cell line|Anti-infection Compound Library concentration|Anti-infection Compound Library nmr|Anti-infection Compound Library in vivo|Anti-infection Compound Library clinical trial|Anti-infection Compound Library cell assay|Anti-infection Compound Library screening|Anti-infection Compound Library high throughput|buy Antiinfection Compound Library|Antiinfection Compound Library ic50|Antiinfection Compound Library price|Antiinfection Compound Library cost|Antiinfection Compound Library solubility dmso|Antiinfection Compound Library purchase|Antiinfection Compound Library manufacturer|Antiinfection Compound Library research buy|Antiinfection Compound Library order|Antiinfection Compound Library chemical structure|Antiinfection Compound Library datasheet|Antiinfection Compound Library supplier|Antiinfection Compound Library in vitro|Antiinfection Compound Library cell line|Antiinfection Compound Library concentration|Antiinfection Compound Library clinical trial|Antiinfection Compound Library cell assay|Antiinfection Compound Library screening|Antiinfection Compound Library high throughput|Anti-infection Compound high throughput screening| partially embedded by an S-180 tumor mass and on day 24, the sciatic nerve was almost surrounded by the S-180 tumor mass. As shown in Fig. 2B, among the three groups studied selleck chemicals (1 × 107, 5 × 106 and 2 × 106 injected groups), neuropathic cancer pain was most steadily induced in 2 × 106 injected
group 2 days after inoculation, suggesting that the suitable cell number that induced neuropathic cancer pain was 2 × 106. Figure 2 A: MRI scans of S-180 tumor mass around the sciatic nerve. After inoculation of S-180 tumor cells around the sciatic nerve, MRI scan was performed. (a) On inoculation day (b) 10 days after inoculation (c) 16 days after inoculation (d) 24 days after inoculation. B: S-180 implantation around sciatic nerve-induced neuropathic cancer pain according to cell number in a time
course study. Withdrawal latency of left hind paws was measured every 2 days until 17 days after inoculation. Values are expressed means ± SE. Statistically significant differences were recorded after comparison to the control using the student’s t test (* p < 0.05, ** p < 0.01). Effect of EA treatment on neuropathic cancer pain TCL As shown in Fig. 3A, EA treatment significantly attenuated paw lifting latency induced 3 days after inoculation by the von Frey test. As shown in Fig. 3B, hind paw-lifting in the tumor control group became apparent when compared to the normal group from day 5 after tumor inoculation and the cumulative paw-lifting duration reached a peak on day 9 where all the mice in the tumor control group showed a slight foot drop in the left hind limb. On the contrary, EA treatment significantly reduced cumulative lifting duration compared to the untreated tumor control group. Effect of EA treatment on substance P and β-endorphin Nine days after inoculation, immunohistochemistry was performed using antibodies against substance P, in sections of spinal cord dorsal horn of mice. As shown in Fig. 4A, substance P was overexpressed in the tumor control group compared to that of the normal control, suggesting that the tumor mass could activate neuropathic pain-related proteins.