Proc Natl Acad Sci USA 1992,89(7):2713–2717.PubMedCrossRef 25. Francisco JA, Stathopoulos C, Warren RA, Kilburn DG, Georgiou G: Specific adhesion and hydrolysis of cellulose by intact Escherichia coli expressing surface anchored cellulase or cellulose binding domains. Biotechnology (N Y) 1993,11(4):491–495.CrossRef 26. Charbit A, Boulain JC, Ryter A, CBL-0137 Hofnung M: Probing the topology of a bacterial membrane protein by genetic insertion of a foreign epitope; expression at the cell surface. EMBO J 1986,5(11):3029–3037.PubMed 27. Francisco JA, Campbell R, Iverson BL, Georgiou G: Production and fluorescence-activated cell sorting of Escherichia coli
expressing a functional antibody fragment on the external
surface. Proc Natl Acad Sci USA 1993,90(22):10444–10448.PubMedCrossRef 28. Charalambous BM, Keen JN, McPherson MJ: Collagen-like sequences GSK690693 purchase stabilize homotrimers of a bacterial hydrolase. Embo J 1988,7(9):2903–2909.PubMed 29. Erickson PR, Herzberg MC: A collagen-like immunodeterminant on the surface of Streptococcus sanguis induces platelet aggregation. J Immunol 1987,138(10):3360–3366.PubMed 30. Erickson PR, Herzberg MC: Purification and partial characterization of a 65-kDa platelet aggregation-associated Tozasertib purchase protein antigen from the surface of Streptococcus sanguis. J Biol Chem 1990,265(24):14080–14087.PubMed 31. Schalen C, Gebreselassie D, Stahl S: Characterization of an erythromycin resistance (erm) plasmid in Streptococcus pyogenes. Apmis 1995,103(1):59–68.PubMedCrossRef 32. Sambrook J, Fritsch EF, Maniatis T: Molecular cloning: a laboratory manual. Cold Spring Harbor, N.Y.: Cold Spring Harbor Laboratory; 1989. 33. Chiang-Ni C, Tsou CC, Lin YS, Chuang WJ, Lin MT, Liu CC, Wu JJ: The transcriptional Demeclocycline terminator sequences downstream of the covR gene terminate covR/S operon transcription to generate covR monocistronic transcripts in Streptococcus pyogenes. Gene 2008,427(1–2):99–103.PubMedCrossRef 34. Liu CZ, Hur BT, Huang TF: Measurement of glycoprotein IIb/IIIa blockade by flow cytometry with fluorescein isothiocyanate-conjugated crotavirin, a member of disintegrins. Thromb
Haemost 1996,76(4):585–591.PubMed 35. Tsai PJ, Kuo CF, Lin KY, Lin YS, Lei HY, Chen FF, Wang JR, Wu JJ: Effect of group A streptococcal cysteine protease on invasion of epithelial cells. Infect Immun 1998,66(4):1460–1466.PubMed 36. Fountoulakis M, Gasser R: Proteomic analysis of the cell envelope fraction of Escherichia coli. Amino Acids 2003,24(1–2):19–41.PubMed Authors’ contributions SMC, YST, and PJT designed the study and wrote the paper. SKL helped draft the manuscript. LCW, CSC and YHL participated in strain construction, RT-PCR, protein purification, antibody generation, cell adhesion assays and FACS analysis. CMW carried out the electron microscopy. All authors read and approved the final manuscript.