Later, penicillin-susceptible S. pneumoniae grew from both samples and its serotype was 4. These results selleck chemicals llc indicated that he had developed pneumococcal bacteremia and meningitis. To confirm the virulence of the isolate strain, KL-B, from the blood sample, we studied the bacteriological and survival examinations in vivo. A murine pneumococcal airway infection
model was induced by inoculating KL-B or S. pneumoniae ATCC BAA-334 as a control to a male 8-week-old CBA/J mouse transnasally as described previously.1 In survival examination, BAA-334-inoculated mice at 1 × 108 cfu/mouse did not die within the observation period; however, KL-B-inoculated mice began to die 2 days later and all of the mice died within 5 days despite of fewer bacteria (n = 4– 6, Figure 1). For bacteriological
examination of the lung and the blood, the mice were sacrificed 48 hours after inoculation. The number of viable bacteria in the lung of KL-B-inoculated mice at 1 × 107, 1 × 106, and 1 × 105 cfu/mouse were 6.57 ± 1.04, 5.71 ± 1.20, and 6.51 ± 1.41 log10cfu/lung (n = 4 − 7,mean ± SD ), respectively. In contrast, no bacteria grew in BAA-334-inoculated groups. Overall, 75% of KL-B-inoculated mice were positive for blood KU-60019 culture. Invasive pneumococcal disease is often observed among persons with underlying conditions such as splenic dysfunction, liver cirrhosis, congestive heart failure, renal failure, and malignancy.2 Among them, splenic dysfunction is the most important risk factor of invasive pneumococcal diseases. Overall incidence of invasive pneumococcal disease was approximately 23 per 100,000 per year in the epidemiological study,2 but the incidence in asplenic adults increased Histamine H2 receptor about 10-fold.3 A variety of medical conditions including sickle cell disease, celiac disease, autoimmune diseases, and congenital anomaly can be associated with asplenism or hyposplenism.4 However, it may not always be easy to diagnose functional asplenia because some patients
are asymptomatic.4 Considering rapid progressive clinical course in this case, the patient might have some immunosuppressant conditions including secondary hyposplenism, although we could not infer underlying diseases. As another possibility, his low level of IgG might increase a risk of infection because the patients with hypogammaglobulinemia are susceptible to invasive pneumococcal infection.5 The most common origin of entry in the patients with pneumococcal sepsis was pneumonia; however, there were also a few cases whose origin was from upper respiratory tract, meningitis, or primary bloodstream infection.6 The episode of sore throat can indicate the origin from upper respiratory tract, supported by typical incubation period of respiratory findings. Therefore, we examined the virulence of the isolate with transnasal respiratory infection murine model.