However, the biological functions of HDAC1 in supporting the growth and development of bovine oocytes and embryos are still not fully elucidates. In this study, three siRNAs (si299, si672, and si1272) targeting to HDAC1 mRNA sequence were designed. After transfection into bovine fibroblast cells, si299, the most effective one in HDAC1 knock-down, was selected.
The selected siRNA was microinjected into bovine germinal vesicle (GV) stage oocytes to determine the functions of HDAC1 in the maturation of bovine oocytes. Finally, the siRNA was microinjected into mature oocytes, which were then parthenogenetically activated and cultured in vitro until the blastocyst stage. The rates of cleavage, blastocyst development and acetylation of lysine 14 of H3 (H3K14) state were checked. The results suggest that HDAC1 knock-down in oocytes did not influence the rates of maturation or cleavage of parthenogenetic embryos. However, the rates of blastocyst BAY 73-4506 manufacturer decreased after siRNA microinjection. Furthermore, histone H3K14 acetylation level increased after siRNA microinjection into parthenogenetic embryos.”
“Purpose: The aims of this study were to evaluate the normal range of the dimensions of lacrimal glands in the healthy orbits of Korean subjects and to evaluate their association with clinical findings.
Methods: A retrospective survey of patients who had visited the Korea University Medical Center and had undergone
orbital computed tomographic this website scan was performed. One hundred eighty Korean patients (90 men and
90 women) who were older than 20 years and without orbital disease were included. Patients with trauma to the orbits or any known lacrimal gland disease were excluded. The length AZD9291 and width of the lacrimal gland were measured in axial and coronal sections using the largest image. The primary outcome was a descriptive analysis of the distribution of dimensions in normal orbits. We analyzed the results and identified associations with age, sex, and laterality and compared our results with a previous study on normal white lacrimal gland dimensions.
Results: The mean lacrimal gland axial length was 14.9 mm in the right orbit and 14.7 mm in left orbit. Coronal lengths averaged 20.9 mm in the right orbit and 20.7 mm in the left orbit. The axial width was 4.1 mm in the right orbit and 4.3 mm in the left orbit. The coronal width averaged 3.6 mm in the right orbit and 3.8 mm in the left orbit. A significant inverse linear relationship was observed in both orbits between gland size and age in all dimensions, with the exception of axial width.
Conclusions: This study presents a normal range of Korean lacrimal gland dimensions as measured by computed tomography. This range can aid in differentiating diseased and nondiseased lacrimal glands, especially when used in combination with physical examination. These data may also be useful in clinical trials that involve lacrimal gland size.