Following the full sequencing of the model virus EhV-86 (isolated in 1999 from the English Channel ( Wilson et al., 2005)), the draft

sequencing of Norwegian isolates EhV-99B1 and EhV-163 (isolated from a fjord in 2000 ( Allen et al., 2006 and Pagarete et al., 2012)) and seven further English Channel coccolithovirus isolates CX-5461 ( Nissimov et al., 2011a, Nissimov et al., 2011b, Nissimov et al., 2012a and Nissimov et al., 2012b), we recently isolated four new coccolithoviruses (EhV-18, EhV-145, EhV-156 and EhV-164) from various locations around the UK coast and assessed their genomic content. Here, we present their draft genomes and highlight the homology and heterogeneity of these genomes to the EhV-86 model reference genome. All four viruses were isolated in 2012 from water samples collected from UK coastal locations during the last 15 years.

EhV-18 and EhV-156 originate in samples collected from the English Channel (50°15′N/04°13′W) in 2008 and 2009 respectively, while EhV-145 and EhV-164 originate in samples collected from Lossiemouth (57°72′N/03°29′W) and the Scottish shore of Fife (exact location unknown, estimated 56°26′N/02°63′W) in 2008 and 2009, respectively. Genome sequencing, finishing, and annotation were performed by Cisplatin manufacturer the NERC Biomolecular Analysis Facility in Liverpool, UK. The genomes were sequenced using the Roche 454 FLX pyrosequencing technology platform. Library construction, sequencing, assembly, and annotation were performed as previously described (Nissimov et al., 2011a and Weynberg et al., 2011). Additional gene prediction analysis and functional annotation was performed within the Integrated-Microbial-Genomes-Expert-Review (IMG-ER)

platform (Markowitz et al., 2009). A total of 44,941, 131,363, 58,158, and 72,877 reads were produced and assembled into 32, 852, 277 Fludarabine supplier and 2280 contigs, comprising of 399,651, 397,508, 399,344 and 400,675 bp for EhV-18, EhV-145, EhV-156, and EhV-164, respectively. General genomic features include nucleotide compositions of 40.49%, 39.94%, 40.47%, and 40.11% G + C; and 503, 548, 493 and 510 predicted CDSs for EhV-18, EhV-145, EhV-156, and EhV-164, respectively. EhV-18 and EhV-156 have five tRNAs (Arg, Asn, Gln, Leu and Lys), while EhV-145 and EhV-164 have four (Arg, Asn, Gln, and Ile). EhV-145 and EhV-164 encode 460 and 435 CDSs with identical homologues in the EhV-86 genome, respectively. In contrast, EhV-18 and EhV-156 have just two CDSs each which share complete identity with their EhV-86 homologues. The majority of CDSs unique to each virus genome encode hypothetical proteins of unknown function.