Deeper incisions were performed in the remaining 3 procedures and necropsy confirmed the complete pyloro-myotomy. Mean duration of procedure was 63 minutes (range 53-75). No mucosal injury was seen. In one case intact serosa was seen but no perforations were noted. After POP the ease of scope passage improved from a mean score of 3.8 to 1.6. Non consequential MP injury was seen in 2/5 cases. Per Oral Pyloro-myotomy (POP) is a feasible procedure and we report first experience with this technique. Future animal lab data and survival models are required to further validate this technique. “
“Recently, cell-based therapies, regenerative medicine, and tissue engineering Trametinib mouse have been progressing rapidly. We have developed

a novel strategy for regenerative medicine to recover tissue functions using temperature-responsive cell culture surfaces. To overcome of conventional methods such as the usage of single-cell suspension injection, we have applied transplantable cell sheets fabricated with temperature-responsive culture surfaces for cell delivery. In the field of gastroenterology, these regenerative medicine and tissue engineering approaches have Talazoparib cost attempted to prevent postoperative stricture by structurally and functionally reconstructing normal tissues through the promotion of early re-epithelialization after endoscopic large size mucosal

resection. Our group previously reported a method of regenerative therapy C1GALT1 involving the transplantation of fabricated autologous oral mucosal epithelial cell sheets in a canine model and demonstrated its human clinical application. So far, the endoscopic technique of cell sheet transplantation was not easily procedure, and there were no endoscopic delivery devices to be useful for cell sheets transplantation. Presently, we are developing a novel endoscopic device for cell sheets transplantation, and we also show recent our research for esophageal regeneration

using cell sheet engineering after circumferential endoscopic large size mucosal resection. We examined allogeneic epidermal cell sheet transplantation using a novel endoscopic delivery device in order to transplant more than one cell sheet at the same time in porcine. The novel device were designed with a computer-aided design system, and the three-dimensional data were transferred to a 3D printer. The surface of the cell sheet transplantation device was fabricated using FDA-sanctioned acrylic material. And then, primary epidermal cells were isolated from the lower abdominal skin of pigs, cultured for 18 days at 37°C on temperature-responsive culture inserts. Transplantable cell sheets were harvested from the inserts by reducing temperature to 20°C. Immediately after creating full circumferential esophageal endoscopic submucosal dissection (ESD), allogeneic epidermal cell sheets were endoscopically transplanted to the ulcer site using a delivery device. The pigs were sacrificed 2 weeks after transplantation.