Conidia gray-green. Colonies grown on SNA in darkness with intermittent light forming conidia within 48 h selleck kinase inhibitor at 35°C; conidia forming at 25°C in light only within 1 week, mainly where the agar had been cut. On SNA conidia forming in small pustules, < ¼ mm diam, individual
conidiophores visible within pustules; pustules often becoming confluent and forming continuous lawns of conidia. Pustules formed of intertwined hyphae; hyphae terminating in sterile hairs and producing conidiophores. Sterile hairs straight, projecting beyond the pustule surface, septate. Conidiophores click here arising laterally from intertwined hyphae, typically constituting 3–5 levels of paired fertile branches, longest fertile branches nearest the conidiophore base, solitary phialides produced near the tip; fertile branches producing phialides directly or often producing paired secondary branches; secondary branches longest near the branching point and reduced to single phialides near the tip of the conidiophore; phialides appearing to be held in whorls; intercalary phialides common (Fig. 8i). Phialides (n = 179) lageniform, (3.7–)5.0–8.0(−11.5) μm long, (2.2–)2.7–3.5(−4.9) μm at the widest point, (1.0–)1.7–2.5(−3.2) μm at the base, L/W (1.1–)1.6–2.9(−4.2), arising from a cell (1.5–)2.5–3.2(−5.0) Ro 61-8048 price μm wide. Conidia (n = 180) ellipsoidal to nearly oblong, (2.7–)3.0–5.0(−7.2) × (1.5–)2.0–2.7(−3.5) μm, L/W (1.2–)1.5–2.1(−2.8) (95% ci: 4.0–4.2 × 2.3–2.4 μm,
L/W 1.7–1.8), green, smooth. Chlamydospores abundant, subglobose, terminal and intercalary, often in pairs. Etymology: ‘flagellatum’ refers to the long hairs that protrude from the pustule. Habitat: endophytic in roots of Coffea arabica. Known distribution: Ethiopia. Holotype: Ethiopia, locality and date not known, isolated from surface-sterilized roots of
Coffea arabica, T. Mulaw (BPI 882293; ex-type culture C.P.K. 3525 = G.J.S. 10–164 = CBS 130626). Sequence: tef1 = FJ763184. Additional cultures examined. Ethiopia, all Exoribonuclease isolated from surface-sterilized roots of Coffea arabica: C.P.K. 3334 = G.J.S. 10–156, sequences: tef1 = FJ763149, chi18-5 = JN258684, rpb2 = JN258688. C.P.K. 3503 = G.J.S. 10–158, sequence: tef1 = FJ763179. C.P.K. 3522 = G.J.S. 10–161, C.P.K. 3523 = G.J.S. 10–162 = CBS 130754, C.P.K. 3524 = G.J.S. 10–163, sequence: tef1 = FJ763183. Additional cultures not analyzed morphologically: Ethiopia, isolated from surface-sterilized roots of Coffea arabica, C.P.K. 3350, sequences: tef1 = FJ763163, chi18-5 = JN258686. C.P.K. 3345, sequences: tef1 = FJ763158, chi18-5 = JN258685, rpb2 = JN258689. Comments: Trichoderma flagellatum is common as an endophyte in roots of coffee in Ethiopia. It forms a clade with T. sinense, T. konilangbra and the new species T. gillesii (Druzhinina et al. 2012). These species are known only from Paleotropical/Asian areas, including East Africa (T. flagellatum, T. konilangbra), the Indian Ocean (T. gillesii) or Taiwan (T. sinense). Apart from T.