All other unlabeled chemicals and reagents were analytical graded. A. bisporus (AB) were commercially purchased from Cuddalore in vegetable markets, Tamil Nadu. A voucher specimen (No. 217) was deposited in Department of Botany, Annamalai University. Powder of AB (50 g) were extracted by stirring with 500 ml of ethanol (30 °C) at 150 rpm for 24 h

and filtered through Whatman No. 4 filter paper. The residues of ethanol extract was then rotary evaporated at 40 °C to dryness, re-dissolved in ethanol to a inhibitors concentration of 10 mg/ml and stored at 4 °C for further use. The terpenoids content of the A. bisporus extracts were determined by the method of Puncal D Test. The flavonoid content of the sample were detected with few ml of ammonia shows the presence of fluorescence OTX015 ic50 this website at 366 nm indicates the presence of flavonoids. The steroids content of the sample were detected by added a few ml of concentrated sulfuric acid solution to the extract. Formation of green color indicates the presence of steroids. The Carbohydrates and Sugars content of the sample were detected by added a few ml of concentrated sulfuric acid solution to the extract and heated formation of charring indicates the presence of carbohydrates. The alkaloids content of the sample were detected by the method of Dragandorff’s test. The

proteins content of the sample were detected by the method of Ninhydrin test. The Tannins content of the sample were detected by 1 ml of

Aluminum chloride. The total phenolic concentration in ABE and ABCNPs was expressed as gallic acid equivalents and was measured according to the method described by Bandoniene et al8 with slight modifications. The Total flavonoid contents (TFC) of the A. bisporus were extracted with 5% NaNO2, 10% AlCl3 and 1 M NaOH were measured at 510 nm with a known quercetin concentration as a standard. The results were expressed as milligrams of quercetin equivalents (CE) per gram of sample. AB loaded chitosan nanoparticles were synthesized by ionic gelation method using tripolyphosphate as a gelating agent. A known amount of chitosan was dissolved in 1% (v/v) acetic acid and allowed to stir for 1 h 3 mg/ml AB ethanol GPX6 extract have prepared already was then added to the freshly prepared chitosan dispersion. The pH of the medium was maintained at 5.0 using 1 M NaOH and then further stirred for 1 h. Finally, 1 mg/ml of TPP was added to the chitosan- AB ethanol extract under mild magnetic stirring. The resulting mixture was allowed to stir for 2 h to form AB encapsulated chitosan nanoparticles. The AB loaded chitosan nanoparticles were collected after the centrifugation of 10,000 rpm for 45 min with 4 °C.9 The powdered samples were collected with the help of lyophilizer and stored at 4 °C for further use. The ABE and ABCNPs were used for analyzing their DPPH radical scavenging activities where determined by the method of Chen.