Additionally, alternative synthetic drugs produced are very expensive, produce adverse side effects and therefore, an alternative approach is needed for formulating ayurvedic drugs having potent anti-bacterial properties. Recent finding confirms Jatiphaladi Churna as having strong anti-bacterial activity in inhibiting and preventing

chronic enteric bacterial infections using disc diffusion method. 27 Currently no reported analytical validation data is available which can be further carried for routine quality control analysis in formulation for this Churna. The analytical separation technique validated in this paper demonstrates for the very first time quantification and separation of eugenol (anti-bacterial and antioxidant) phytochemical from Jatiphaladi Churna with very short retention time (Fig. 3D). This finding can be further used for critical simultaneous Verteporfin datasheet quantification of other marker compounds such as active markers (possess

therapeutic activity) from Jatiphaladi Churna and other marketed herbal medicines, thus facilitating ABT-199 cell line easy separation and detection of phytochemicals for development of herbal medicines against multidrug resistant microbial pathogens. Eugenol present in clove oil has been proved to possess anti-microbial activity against bacteria species such as S. aureus ATCC25923, K. pneumoniae species, etc. 28 Gas chromatography mass spectrophotometer (GC–MS) technique has been used for detection of eugenol. In principle, the main shortcomings of this technique for quantification of phytochemical are that the results are not of very high resolution, difficult to record and not automated. GC–MS operates at high temperature and this may

affect the stability of thermally labile phytochemical constituents in herbal formulations. On the other hand, validated RP-HPLC method developed in this paper for detection of detection of eugenol was found to be highly sensitive and flexible technique. This was evident from Ruggedness validation parameter data, in which chromatographic conditions such as Mobile phase concentration and Flow rate change were deliberately changed Urease without use of any heating protocols and need of high temperature. The retention time recorded completely satisfy the acceptance criteria ±1% ( Table 2). Thus, the validated analytical chromatographic method reported is highly rugged, sensitive, requires less retention time and is not affected by minuscule changes in the chromatographic conditions (Fig. 4F). Fishes get easily get spoilt at room temperature and therefore, increasing the lifespan of fishes is now big issue in food technology industry.29 Eugenol has scientifically been proven to have anti-microbial activity because of it significant anti-oxidant capacity and has currently acquired large interest among food scientists to incorporate this phytochemical as natural anti-microbial agent in the form of natural preservative in extending shelf life of fishes.