[81]. Estimation of shear stress Shear stress (τ) is defined as:τ = μ(dv/dy) where μ is the absolute (dynamic) viscosity (approximately 10-2 dynes sec cm-2). For a cylindrical geometry the slope of the velocity profile at the tube wall (dv/dy) is related to the maximum velocity (Vmax) by:dv/dy = 2(Vmax/r) where r is the radius of the tubing and:Vmax = 2 V where V is the mean flow velocity across the velocity profile (the volumetric flow divided by the cross sectional area of the interior of the tubing). The shear stress applied in draining the tubing was estimated from the average V determined from the time required for

the medium plug to reach the end of the tubing (0.5s). Acknowledgements This work was supported by a grant from NIH (1 R21 GM070554-01A1) to P.A.S We are grateful to Aaron Mitchell, Clarissa Nobile, Frank BIBW2992 price Smith, Bruce Granger, Jennifer Carbrey, Paola Zucchi and Carol Kumamoto for generously providing us with mutant strains. We are grateful to Jean-Sébastien Deneault and CFTRinh-172 the members of the BRI microarray lab for technical help. We also would like to thank Hervé Hogues for bioinformatic assistance. We thank Mark Young and Trevor Douglas at MSU for their

intellectual and monetary (CBIN) support. This is NRC publication number 49572. Electronic supplementary material Additional file 1: Biofilm Time Course Array Dataset. Complete list of differentially regulated genes (ZIP 4 MB) Additional file 2: Biofilm versus Batch

Time Array Dataset. Complete list of differentially regulated genes (ZIP 4 MB) Additional file 3: Primers used in this study. Primer sequences used to construct the mutant strains (DOC 75 KB) References 1. Fridkin SK, Jarvis WR: Idasanutlin mw Epidemiology of nosocomial fungal infections. Clinical Microbiology Reviews 1996, 9:499–511.PubMed 2. Eggimann P, Cepharanthine Garbino J, Pittet D: Epidemiology of Candida species infections in critically ill non-immunosuppressed patients. Lancet Infectious Diseases 2003, 3:685–702.CrossRefPubMed 3. Tan LH, Sun XN, Zhu XK, Zhang ZW, Li PH, Shit Q: Epidemiology of nosocomial pneumonia in infants after cardiac surgery. Chest 2004, 125:410–417.CrossRefPubMed 4. Voss A, leNoble J, Lunel FMV, Foudraine NA, Meis J: Candidemia in intensive care unit patients: Risk factors for mortality. Infection 1997, 25:8–11.CrossRefPubMed 5. Macphail GLP, Taylor GD, Buchanan-Chell M, Ross C, Wilson S, Kureishi A: Epidemiology, treatment and outcome of candidemia: a five-year review at three Canadian hospitals. Mycoses 2002, 45:141–145.CrossRefPubMed 6. Alonso-Valle H, Acha O, Garcia-Palomo JD, Farinas-Alvarez C, Fernanez-Mazarrasa C, Farinas MC: Candidemia in a tertiary care hospital: Epidemiology and factors influencing mortality. Eur J Clin Microbiol Infect Dis 2003,22(4):254–257.PubMed 7.