6A). Forkhead box A2 (Foxa 2 or HNF3-beta), undetectable in control mouse MSCs, could be readily detected after the addition of NECA (Fig. 6B). Foxa2 has been reported to have a key role on the differentiation of bone marrow MSCs into hepatocyte-like cells.26 Furthermore, NECA increased the expression of Goosecoid (GSC) (Fig. 6C). GSC is important for the development

of mesentoderm and definitive endoderm in the mouse embryo.27, 28 NECA was not able to induce other endodermal or hepatocyte-specific genes, such as Sox17, alpha-fetoprotein (AFP), albumin, epithelial gene adhesion molecule (EpCAM), or tyrosine aminotransferase (TAT), in the mouse MSC (Fig. 6D-H). We found that NECA induces the expression of GSC and Sox 17 in human MSCs (Fig. 7A, B). Both genes are critical for the development of definitive endoderm (the precursors of PD332991 Akt inhibitor hepatocytes) during embryogenesis.29 Also, NECA induced the expression of EpCAM, which is a key marker of hepatic stem cells and hepatoblasts.30 Furthermore, NECA induced the hepatocyte-specific genes albumin TAT in human MSCs (Fig. 7C-E). However, it did not induce

the expression of AFP, Foxa1, or Foxa2 in human MSCs. Mesenchymal stem cells (MSCs) are multipotential and capable of differentiation into numerous connective tissue lineages, as well as cells of endodermal origin.2–4 This, along with ease of isolation and capacity to undergo extensive replication in vitro, have made MSCs candidates for cell-based tissue engineering approaches.31 In an animal model of liver injury, transplanted MSCs differentiated into functioning hepatocyte-like cells and ameliorated liver injury.4 The mechanisms of localization to sites of injury and differentiation into hepatocyte-like cells are not well understood. Migration is thought to be mediated largely by soluble factors released from platelets

and other cell types, sustaining chemotaxis, or movement of cells up a gradient of soluble factors.32 The binding of these factors to membrane receptors initiates a series of intracellular molecular events leading to the reorganization of the cytoskeleton into locomotive machinery. Adenosine is produced from the metabolism of purines during the degradation of nucleic acids of apoptosing 上海皓元 cells and is rapidly metabolized by adenosine deaminase. The extracellular concentrations of adenosine rise rapidly in tissue injury from the 0.1-0.3 μM range to greater than 100 μM. Such rapid metabolism limits the half-life to a few minutes. Because adenosine levels are highest in the immediate microenvironment of cellular injury, we were interested in examining whether adenosine has a functional affect of MSC migration and differentiation. Messenger RNA for A2a and A2b receptors was expressed in mouse MSCs and A1, A2a, and A2b in human MSCs (Fig. 1A, B). Interestingly, adenosine did not induce chemotaxis but rather inhibited the well-known chemoattractant HGF.