5 Hz, benzylic), 4.14 (m, 2H, 2× –OCH), 3.69 (s, 3H, 2× –OCH3) 1.98–1.81 (m, 4H, 2× –CH2), VRT752271 manufacturer 1.81–1.68 (m, 4H, 2× –CH2), 1.28 (d, 6H, J = 6.4 Hz, 2× –CH3); 13C NMR (75 MHz, CDCl3): 166.2, 158.3, 145.2, 129.1, 128.8, 120.2, 113.2, 79.8, 72.2, 66.5, 55.4, 39.3, 28.2, 21.3; IR (neat): 3068, 2932, 2859, 1722, 1608, 1527, 1462, 1427, 1273, 1105, 918, 702 cm−1. To a solution of 19 (96 mg, 0.16 mmol) in aq. CH2Cl2 (2 mL, 19:1), DDQ (57 mg, 0.24 mmol) was added and stirred at room temperature

for 3 h. The reaction mixture was quenched with sat. NaHCO3 solution (1 mL), filtered and washed with CH2Cl2 (10 mL). The filtrate was washed with water (3 mL), brine (3 mL), dried (Na2SO4) and evaporated under reduced pressure to furnish 7 (43 mg, 81%) as a white solid. m.p.: 124–126 °C; [α]D +13.2 (c 0.11, CHCl3); 1H NMR (CDCl3, 300 MHz): δ 6.91 (dd, 2H, J = 15.3, 5.3 Hz, olefinic), 5.89 (dd, 2H, J = 15.3, 1.6 Hz, olefinic), 5.16–5.07 (m, 2H, 2× –OCH), 4.31–4.18 (m, 2H, 2× –OCH), HDAC inhibitor 2.18 (br. s, 2H, 2× –OH), 1.98–1.83 (m, 4H, 2× –CH2), 1.81–1.68 (m, 4H, 2× –CH2), 1.12 (d, 6H, J = 6.4 Hz, 2× –CH3); 13C NMR (75 MHz, CDCl3): δ 168.4,

147.2, 120.8, 73.9, 69.8, 30.3, 29.2, 19.6; IR (neat): 2972, 2922, 2853, 1730, 1462, 1126, 835 cm−1; All authors have none to declare. “
“An increase in severe opportunistic fungal infections that threaten public health is apparent.1 This is associated with the wide-spread use of broad-spectrum antibiotics as well as immunosuppressive,

anticancer, and antiretroviral drugs2, 3 and 4 causing resistance against current antifungal drugs. Candida albicans is present in the gut of about 80% of the human Non-specific serine/threonine protein kinase population and is a major opportunistic pathogen. 5 The high incidence of acquired immune deficiency syndrome (AIDS) in sub-Saharan Africa facilitated this fungus to become a major source of health problems in these developing countries. 2, 3 and 4 The deficiency of health care clinics adequately equipped to treat patients in Southern Africa further contribute towards the problem of drug resistance. Many of these patients revert to traditional healers who use medicinal plants to treat Candida infections. 6 Medicinal plants are good sources of potential antifungal drugs. 7 Homoisoflavanone-containing plants have been used in the past by traditional healers to treat fungal and other skin infections.7, 8, 9 and 10 Isolated homoisoflavanones have also been reported to possess antifungal activity.11, 12 and 13 Structurally homoisoflavanones are similar to isoflavonoids. Isoflavonoids have a fifteen-carbon atom skeleton whilst inhibitors homoisoflavonoids have sixteen carbon atoms. Four types of homoisoflavanones can be distinguished, namely 3-benzyl-4-chromanones, 3-benzylidene-4-chromanones, 3-benzyl-3-hydroxy-4-chroma-nones and scillascillins.14 The 3-benzylidene-4-chromanones exhibits antifungal activity.