, 2006 and Head et al., 2009). We were thus able to remove this confounding factor in our study by adapting a human model using ECG electrodes (Fregni et al., 2006c). For sham stimulation, the electrodes were placed in the same positions as for real stimulation; however, SB431542 in vitro the stimulator was turned off after 30 s of stimulation so the animals could maintain continuity
of the physical sensation of real tDCS conditions (Gandiga et al., 2006). Forty-eight hours after tDCS treatment, the animals were killed by decapitation. Trunk blood was collected and centrifuged at 5000 g for 5 min at room temperature. Animals were killed by an experienced investigator. Serum and hippocampus Dasatinib supplier were frozen at −70 °C for subsequent analysis. Serum interleukin-1 and corticosterone levels were determined using commercially available enzyme-linked
immunosorbent assay (ELISA) kits for rat interleukin-1 (Uscn Life Science Inc.) or corticosterone (IBL Corticosterone Kit), according to manufacturer instructions. The results are expressed in pg/mL and nmol/L, respectively. TNF analysis was performed on hippocampus homogenates. TNF levels were measured by a commercially available enzyme-linked immunosorbent assay (ELISA) kit for rat tumor necrosis factor-alpha (Uscn Life Science Inc.), according to manufacturer protocols. The results are expressed in pg/mL. The results are presented as the mean±standard error of the Selleck Osimertinib mean (SEM). As data were normally distributed, we assessed the difference between groups
using one-way ANOVA with Tukey’s test when necessary. P-values less than 0.05 were considered significant. This research was supported by the following Brazilian funding agencies: National Council for Scientific and Technological Development—CNPq (I.L.S. Torres, W. Caumo); Committee for the Development of Higher Education Personnel—CAPES (J.R. Rozisky, A. Souza, G. Laste, L.N.S. Adachi, L.F. Medeiros); Graduate Research Group (GPPG) of Hospital de Clínicas de Porto Alegre—HCPA (I.L.S. Torres – Grant 100381). Our acknowledgments to engineering from the HCPA for have developed the tDCS stimulator. “
“It is with deep regret we acknowledge the death of Psychology professor J. Philippe Rushton from the University of Western Ontario, London, Canada. Rushton died peacefully October 2nd 2012 from Addison’s Disease, a condition compromising the immune system. His death marks the day when Psychology, Behavior Genetics, Genetic Similarity, Life History Theory, and Evolutionary thinking lost one of their prominent sons. Rushton leaves behind him his brother Peter Rushton, son Stephen Philippe Rushton and daughter Katherine Vanderzwet, and grandchildren Jasmine, Aundreia, and great-granddaughter Paige.