Figure 5 Stability analysis of various VipA mutants and their effect

on VipB stability. Left panel: The intrabacterial stability of His6-tagged VipA mutants was examined. At time 0, chloramphenicol was added to stop new protein synthesis. Samples from pelleted bacteria were taken at different time points, and the amount of VipA protein was detected by western blot using anti-His antibodies. Right panel: The impact on VipB expression/stability exhibited by the various vipA mutants was investigated by western blot using anti-VipB antibodies. VipA/VipB complex formation influences the ability of V. cholerae to compete with E. coli Lately, type VI secretion (T6S) has been shown to play an important role in interbacterial interactions, more specifically in bacterial killing and competition [16–20]. For example, BIX 1294 solubility dmso V. learn more cholerae V52 uses its T6SS to efficiently kill E. coli[21], which in turn requires most of the T6S genes including vipA and vipB[20]. V. cholerae A1552 also uses T6S to compete with E. coli, although it does not exert the massive T6S-mediated killing exhibited by strain V52 [13]. To investigate the ability of the A1552 vipA mutants to compete

with E. coli, we used a previously established competition assay that involves mixing V. cholerae and E. coli MC4100, coculturing them on filters on agar plates at T6SS inducing conditions (i.e. high salt, 37°C) for 5 h, and then recovering the click here number of surviving target cells [13]. In addition to parental A1552 and ΔvipA, two categories of vipA mutants were used in the assay: 1) single substitution mutants D104A, V106A, V110A and L113A, which all showed slightly decreased binding to VipB, although without any obvious defects in VipB stability or Hcp secretion, and 2) multiple substitution mutants D104A/V106A, V110A/L113A, D104A/V106A/V110A and Farnesyltransferase D104A/V106A/V110A/L113A, which all showed null

phenotypes with respect to VipB binding, VipB stability and Hcp secretion. When E. coli was cocultured with parental A1552, there was a 2 log10 drop in the number of viable E. coli cells recovered compared with results for cultures inoculated with medium alone (Figure 6). However, since the numbers of viable E. coli never dropped below the initial inoculum, this suggests that A1552, in contrast to the highly bactericidal strain V52, may not be able to effectively kill the target cells. This may likely be explained by the observation that V52, in contrast to A1552, encodes a constitutively active T6SS that secretes high amounts of Hcp and other effector proteins [12]. Using the identical set-up, V52 was shown to efficiently kill E. coli, as the initial bacterial numbers dropped by > 1,000-fold (data not shown). The bacterial competition exerted by strain A1552 was shown to depend on a functional T6SS, since the number of E. coli increased by ~ 1.5 log10 when cocultured with the ΔvipA mutant compared to parental A1552 (Figure 6).