Serum from HCC patients contained higher concentration TGF-β1 than those from cirrhosis and healthy control. Flow cytometry indicated that blocking TGF-β1 stimulated HCC cells proliferation and knockdown it leaded to G1 arrest. Colony formation assay displayed that silencing TGF-β1 affect proliferation of cancer cells. And animal experiments showed that interference TGF-β1 decreased the metastatic nodules in lungs.

Conclusion: Cancer secreted-TGF-β1 is necessary for proliferation of HCC cells. Knockdown Trichostatin A mouse TGF-β1 could inhibit tumor growth and decrease metastases. The findings suggest that TGF-β1 could be a potential therapeutic target for HCC treatment. Key Word(s): 1. HCC; 2. TGF-β1; 3. metastasis; Presenting Author: HIROKI UCHIDA Additional Authors: YUKIO IWASHITA, KIMINORI WATANABE, TAKAHIDE KAWASAKI, YUICHIRO KAWANO, YOKO KOMORI, KAZUHIRO YADA, MASAYUKI OHTA, SEIGO KITANO Corresponding Author: HIROKI UCHIDA Affiliations: Oita university Objective: Surgical resection for hepatocellular carcinoma (HCC) is regarded as a curable treatment. A tumor adhering to the major hepatic vessels sometimes causes a need to perform hepatectomy without surgical margin. The aim of this study was to evaluate the short-term

outcomes INCB024360 datasheet of hepatectomy with null surgical margin for HCC. Methods: rom January 2010 to December 2012, 70 patients who underwent curative hepatectomy for HCC in Oita University were analyzed. They were divided into two group with null margin group and negative margin group. These two groups were compared in terms of clinicopathological characteristics, perioperative features, and short-term outcomes. 上海皓元 Results: Nineteen patients (27.1%) had been performed hepatectomy with null margin diagnosed pathologically. Mean intraoperative

blood loss was greater and operation time was longer in the null margin group as compared to those in the negative margin group. There were no significant differences in overall survival, disease free survival and other clinicopathological features between the two groups. In the null margin group, 12 patients had recurrence (59.1%) and the recurrence site was liver in all the patients. Four of the 12 patients (33%) had recurrence at the resected stump of the liver and the mean period of recurrence was 10.8 months. Conclusion: Hepatectomy with null surgical margin for HCC is technically demanding. It would lead to comparable and adequate surgical outcomes in patients with tumors in contact with major vessels, compared to hepatectomy with negative surgical margin. Key Word(s): 1. HCC; 2.