l. is also traditionally considered to be highly polymorphic (Jumpponen & Trappe, 1998; Gams, 2000). Likewise, there are still some disagreements between the morphological and the molecular identification of Phialophora spp. (Yan et al., 1995; de Hoog et al., 1999; Ulrich et al., 2000; Sieber, 2002). Species formerly classified in the genus are now known to belong to different orders of Ascomycetes. Gams (2000) began to sort out the taxonomy of Phialophora spp. and erected Harpophora for anamorphs of Gaeumannomyces and Magnaporthe within the Magnaporthaceae. Its morphological characteristics include fast-growing, thin colonies with ‘runner hyphae’ and more or less pigmented phialides coupled with cylindrical,

hyaline selleck chemicals and strongly curved conidia. Up to now, four species combinations have been described within Harpophora, i.e. Harpophora radicicola (type species, previously Phialophora radicicola) (McKeen, 1952; Walker, 1980), Harpophora maydis (Cephalosporium maydis) (Samra et al., 1963), Harpophora graminicola (Phialophora graminicola) (Hornby et al., 1977; Walker, 1980) and Harpophora zeicola (Phialophora zeicola) (Deacon & Scott, 1983). In addition, the anamorphs of Gaeumannomyces spp. belong here without being separately named as anamorph species. We have recently started an

examination of the endophytic fungal community in wild rice (Oryza granulata) roots in China, during which we found a new species, which is described here as Harpophora oryzae.

The site of study is located in Xishuangbanna, Yunnan http://www.selleckchem.com/products/VX-770.html province, southwest of China (22°04′–22°17′N; 100°32′–100°44′E). In September of 2007 and 2008, we collected samples from two sites in Xishuangbanna. Healthy and intact wild rice plants with bulk soil were packed in a box and carefully transported to the laboratory within 48 h. For isolation of endophytic fungi, healthy roots (free of detectable lesions) of the sample rice plants were gently rinsed with tap water, immersed in ethanol (75% v/v) for 30 s, then in sodium hypochlorite (1% w/v) for 10 min and finally rinsed three times in sterile-distilled water. Roots were cut into segments of 0.6 cm length and transferred to a malt extract agar (MEA) Fenbendazole plate containing 2% malt extract and 2% agar (w/v) supplemented with chloramphenicol (50 mg L−1) to prevent bacterial growth. Six root fragments were placed on one plate and incubated at 25 °C in permanent darkness. After the emergence of fungal hyphae, these were cut off and subcultured. Isolates were stored by covering a culture on potato dextrose agar (PDA) slants with sterile liquid paraffin at 25 °C and by preservation in aqueous 15% v/v glycerol additionally containing glucose (10 g L−1), yeast extract (1 g L−1) and casein hydrolysate (1 g L−1) at −70 °C. Light-microscopic analysis was performed using an Olympus BX51 microscope. Images were acquired using axiovision 3.1. For the determination of spore characteristics, specimens were mounted in water.